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Did Fauci even understand what Geartner was explaining to
Posted on: June 2, 2021 at 15:23:19 CT
BH O'bonga MU
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him regarding the genetic engineering of the China Virus back on March 11, 2020?
"Hello Anthony, This is how the virus was created. Intervirion Fusion. HIV-luc(ACE2) (500 ng ofp24) was mixed with 1,000 ng of p24 ofHIV-gfp particles incorporating ASLV-A envelope, SARS-CoV S protein, or both e nvelopes in PBS at 4 °C for 30 min to allow binding. Samples were ra ised to 37°C for 15 min to allow for conformational rearrangements. Virions were adjusted to the desired pH with 0.1 M citric acid. PBS, TPCK-trypsin (final concentration 10 ~tg/ml), CTSL, cathepsin B (CTSB) (final concentrations 2 ~tg/ml) or CTSL buffer alone was then added. Recombinant CTSL (R &D Systems) was preactivated by incubation for 15 min at 10 μg/ml in 50 mM Mes, pH 6.0, on ice. R ecombinant CTSB (R &D Systems) was preactivated i n 25 mM Mes, 5 mM OTT, pH 5.0, for 30 min at 25°C. After a 10-min incubation at 25°C , proteolysis was halted by the addition of 300 μ1 ofDMEMIO containing leupeptin (25 μg/ ml) and STI (75 μg/ml ). Virions were then incubated at 37°C for 30 min to allow membrane fusion. 100 μl of the virion mixture was added in quadruplicate to HeLa-Tva cells pretreated for 1 h with leupeptin (20 ~tg/ml). The ce lls were sp in-infected and incubated at 37°C for 5 h"
https://www.documentcloud.org/documents/20793561-leopold-nih-foia-anthony-fauci-emails
"Hello Anthony, This is how the virus was created. Intervirion Fusion. HIV-luc(ACE2) (500 ng ofp24) was mixed with 1,000 ng of p24 ofHIV-gfp particles incorporating ASLV-A envelope, SARS-CoV S protein, or both e nvelopes in PBS at 4 °C for 30 min to allow binding. Samples were ra ised to 37°C for 15 min to allow for conformational rearrangements. Virions were adjusted to the desired pH with 0.1 M citric acid. PBS, TPCK-trypsin (final concentration 10 ~tg/ml), CTSL, cathepsin B (CTSB) (final concentrations 2 ~tg/ml) or CTSL buffer alone was then added. Recombinant CTSL (R &D Systems) was preactivated by incubation for 15 min at 10 μg/ml in 50 mM Mes, pH 6.0, on ice. R ecombinant CTSB (R &D Systems) was preactivated i n 25 mM Mes, 5 mM OTT, pH 5.0, for 30 min at 25°C. After a 10-min incubation at 25°C , proteolysis was halted by the addition of 300 μ1 ofDMEMIO containing leupeptin (25 μg/ ml) and STI (75 μg/ml ). Virions were then incubated at 37°C for 30 min to allow membrane fusion. 100 μl of the virion mixture was added in quadruplicate to HeLa-Tva cells pretreated for 1 h with leupeptin (20 ~tg/ml). The ce lls were sp in-infected and incubated at 37°C for 5 h"
https://www.documentcloud.org/documents/20793561-leopold-nih-foia-anthony-fauci-emails
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Did Fauci even understand what Geartner was explaining to - BH O'bonga MU - 6/2 15:23:19